JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Biochemistry
/
快速网格准备时间解决低温电子显微镜
JoVE Journal
Biochemistry
Author Produced
A subscription to JoVE is required to view this content.
Sign in or start your free trial.
JoVE Journal
Biochemistry
Fast Grid Preparation for Time-Resolved Cryo-Electron Microscopy
Please note that all translations are automatically generated.
Click here for the English version.
快速网格准备时间解决低温电子显微镜
DOI:
10.3791/62199-v
•
10:05 min
•
November 06, 2021
•
David P. Klebl
,
Frank Sobott
,
Howard D. White
,
Stephen P. Muench
1
School of Biomedical Sciences, Faculty of Biological Sciences & Astbury Centre for Structural and Molecular Biology
,
University of Leeds
,
2
School of Molecular and Cellular Biology, Faculty of Biological Sciences & Astbury Centre for Structural and Molecular Biology
,
University of Leeds
,
3
Department of Physiological Sciences
,
Eastern Virginia Medical School
Chapters
00:10
Introduction
00:43
Preparing the System
04:34
Fast Grid Preparation
07:12
Time-Resolved Cryo-EM
08:46
Representative Results
09:34
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
在这里,我们提供了一个详细的协议,用于快速网格制作和快速混合和冻结的快速网格制作设备,以进行时间解决的实验。
Tags
Cryo-electron Microscopy
Time-resolved
Grid Preparation
Rapid
Protein Concentration
Syringe Pump
Nitrogen Gas
Buffer
Spray Nozzle
Plunging Arm
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
时间分辨电喷雾电离氢氘交换质谱用于研究蛋白质结构与动态
单粒子冷冻电子显微镜在结构中测定脂质敏感分通道 trpc3 的表达与纯化
冷冻电子断层扫描
的糖核
酸的准备和冷冻-FIB微加工
单颗粒冷冻电子显微镜:从样品到结构
微图案透射电子显微镜网格,用于在全细胞冷冻电子断层扫描工作流程中指导细胞定位
强大的单颗粒冷冻电子显微镜 (cryo-EM) 处理工作流程,具有冷冻 SPARC、RELION 和 Scipion
制备与DNA修复因子络合的核小体核心颗粒用于冷冻电镜结构测定
从哺乳动物细胞中提取修饰的微管以通过冷冻电子显微镜研究微管 - 蛋白质复合物
哺乳动物细胞
原位
冷冻断层扫描的样品制备
Cryo-Electron Microscopy Screening Automation Across Multiple Grids Using Smart Leginon
Read Article