Journal
/
Biochemistry
/
Membranlar Arasında Fosfataylserine/Fosfatidiylinositol 4-Fosfat Değişiminin Floresan Bazlı Ölçümleri
JoVE Journal
Biochemistry
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biochemistry
Fluorescence-Based Measurements of Phosphatidylserine/Phosphatidylinositol 4-Phosphate Exchange Between Membranes
Please note that all translations are automatically generated. Click here for the English version.

Membranlar Arasında Fosfataylserine/Fosfatidiylinositol 4-Fosfat Değişiminin Floresan Bazlı Ölçümleri

Türkçe

Automatically Generated

3,620 Views

•

08:49 min

•

March 14, 2021

DOI:

10.3791/62177-v

DOI:
10.3791/62177-v

•

08:49 min
March 14, 2021

•

2 Views
, , ,
1Université Côte d’Azur, Centre National de la Recherche Scientifique, Institut de Pharmacologie Moléculaire et Cellulaire

Summary

Automatically generated

Burada, bir proteinin fosfatidiylserine veya fosfatidiliinositol 4-fosfat in vitro'yuayıklayıp taşımadığını belirlemek için floresan lipid sensörleri ve lipozomlar kullanarak protokolleri açıklıyoruz.

Explore More Videos

FluorescencePhosphatidylserinePhosphatidylinositol 4-phosphateLipid TransferMembraneLiposomesNBD-C2 LactLipid SensorLipid Transfer ProteinNBD-PH FAPP ProbePI(4)P Extraction AssayHEPES Potassium Acetate BufferMagnesium ChloridePhosphatidylcholinePhosphatidylethanolamine

Read Article

Cite this Article

Ikhlef, S., Lipp, N., Magdeleine, M., Drin, G. Fluorescence-Based Measurements of Phosphatidylserine/Phosphatidylinositol 4-Phosphate Exchange Between Membranes. J. Vis. Exp. (169), e62177, doi:10.3791/62177 (2021).

Download .ris file

Copy

Share Video

.

Copy