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Mediciones basadas en fluorescencia del intercambio de fosfatidilserina/fosfatidilinositol 4-fosfato entre membranas
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Biochemistry
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JoVE Journal Biochemistry
Fluorescence-Based Measurements of Phosphatidylserine/Phosphatidylinositol 4-Phosphate Exchange Between Membranes
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Mediciones basadas en fluorescencia del intercambio de fosfatidilserina/fosfatidilinositol 4-fosfato entre membranas

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3,620 Views

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08:49 min

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March 14, 2021

DOI:

10.3791/62177-v

DOI:
10.3791/62177-v

•

08:49 min
March 14, 2021

•

2 Views
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1Université Côte d’Azur, Centre National de la Recherche Scientifique, Institut de Pharmacologie Moléculaire et Cellulaire

Summary

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Aquí, describimos protocolos que utilizan sensores de lípidos fluorescentes y liposomas para determinar si una proteína extrae y transporta fosfatidilserina o fosfatidilinositol 4-fosfato in vitro.

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Ikhlef, S., Lipp, N., Magdeleine, M., Drin, G. Fluorescence-Based Measurements of Phosphatidylserine/Phosphatidylinositol 4-Phosphate Exchange Between Membranes. J. Vis. Exp. (169), e62177, doi:10.3791/62177 (2021).

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