Here, we describe protocols using fluorescent lipid sensors and liposomes to determine whether a protein extracts and transports phosphatidylserine or phosphatidylinositol 4-phosphate in vitro.
Ikhlef, S., Lipp, N., Magdeleine, M., Drin, G. Fluorescence-Based Measurements of Phosphatidylserine/Phosphatidylinositol 4-Phosphate Exchange Between Membranes. J. Vis. Exp. (169), e62177, doi:10.3791/62177 (2021).