Journal
/
Biochemistry
/
Fluorescence-Based Measurements of Phosphatidylserine/Phosphatidylinositol 4-Phosphate Exchange Between Membranes
JoVE Journal
Biochemistry
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biochemistry
Fluorescence-Based Measurements of Phosphatidylserine/Phosphatidylinositol 4-Phosphate Exchange Between Membranes

Fluorescence-Based Measurements of Phosphatidylserine/Phosphatidylinositol 4-Phosphate Exchange Between Membranes

English

Automatically Generated

3,620 Views

•

08:49 min

•

March 14, 2021

DOI:

10.3791/62177-v

DOI:
10.3791/62177-v

•

08:49 min
March 14, 2021

•

2 Views
, , ,
1Université Côte d’Azur, Centre National de la Recherche Scientifique, Institut de Pharmacologie Moléculaire et Cellulaire

Summary

Automatically generated

Here, we describe protocols using fluorescent lipid sensors and liposomes to determine whether a protein extracts and transports phosphatidylserine or phosphatidylinositol 4-phosphate in vitro.

Explore More Videos

FluorescencePhosphatidylserinePhosphatidylinositol 4-phosphateLipid TransferMembraneLiposomesNBD-C2 LactLipid SensorLipid Transfer ProteinNBD-PH FAPP ProbePI(4)P Extraction AssayHEPES Potassium Acetate BufferMagnesium ChloridePhosphatidylcholinePhosphatidylethanolamine

Read Article

Cite this Article

Ikhlef, S., Lipp, N., Magdeleine, M., Drin, G. Fluorescence-Based Measurements of Phosphatidylserine/Phosphatidylinositol 4-Phosphate Exchange Between Membranes. J. Vis. Exp. (169), e62177, doi:10.3791/62177 (2021).

Download .ris file

Copy

Share Video

.

Copy