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Site-Directed φC31-Mediated Integration and Cassette Exchange in Anopheles Vectors of Malaria
JoVE Journal
遺伝学
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JoVE Journal 遺伝学
Site-Directed φC31-Mediated Integration and Cassette Exchange in Anopheles Vectors of Malaria

Site-Directed φC31-Mediated Integration and Cassette Exchange in Anopheles Vectors of Malaria

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09:38 min

February 02, 2021

DOI:

09:38 min
February 02, 2021

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概要

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The protocol describes how to achieve site-directed modifications in the genome of Anopheles malaria mosquitoes using the φC31 system. Modifications described include both the integration and the exchange of transgenic cassettes in the genome of attP-bearing docking lines.

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