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Quantitative Metabolomics of Saccharomyces Cerevisiae Using Liquid Chromatography Coupled with Tandem Mass Spectrometry
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Biochemistry
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JoVE Journal Biochemistry
Quantitative Metabolomics of Saccharomyces Cerevisiae Using Liquid Chromatography Coupled with Tandem Mass Spectrometry

Quantitative Metabolomics of Saccharomyces Cerevisiae Using Liquid Chromatography Coupled with Tandem Mass Spectrometry

English

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3,977 Views

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07:25 min

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January 05, 2021

DOI:

10.3791/62061-v

DOI:
10.3791/62061-v

•

07:25 min
January 05, 2021

•

8 Views
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1Department of Biology,Concordia University, 2Centre for Biological Applications of Mass Spectrometry,Concordia University

Summary

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We present a protocol for the identification and quantitation of major classes of water-soluble metabolites in the yeast Saccharomyces cerevisiae. The described method is versatile, robust, and sensitive. It allows the separation of structural isomers and stereoisomeric forms of water-soluble metabolites from each other.

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Mohammad, K., Jiang, H., Titorenko, V. I. Quantitative Metabolomics of Saccharomyces Cerevisiae Using Liquid Chromatography Coupled with Tandem Mass Spectrometry. J. Vis. Exp. (167), e62061, doi:10.3791/62061 (2021).

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