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Quantitative Metabolomics of Saccharomyces Cerevisiae Using Liquid Chromatography Coupled with Tandem Mass Spectrometry
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JoVE Journal Biyokimya
Quantitative Metabolomics of Saccharomyces Cerevisiae Using Liquid Chromatography Coupled with Tandem Mass Spectrometry

Quantitative Metabolomics of Saccharomyces Cerevisiae Using Liquid Chromatography Coupled with Tandem Mass Spectrometry

3,977 Views

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07:25 min

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January 05, 2021

DOI:

10.3791/62061-v

DOI:
10.3791/62061-v

•

07:25 min
January 05, 2021

•

8 Views
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1Biyoloji Bölümü,Concordia University, 2Centre for Biological Applications of Mass Spectrometry,Concordia University

Özet

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We present a protocol for the identification and quantitation of major classes of water-soluble metabolites in the yeast Saccharomyces cerevisiae. The described method is versatile, robust, and sensitive. It allows the separation of structural isomers and stereoisomeric forms of water-soluble metabolites from each other.

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Mohammad, K., Jiang, H., Titorenko, V. I. Quantitative Metabolomics of Saccharomyces Cerevisiae Using Liquid Chromatography Coupled with Tandem Mass Spectrometry. J. Vis. Exp. (167), e62061, doi:10.3791/62061 (2021).

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