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Quantitative Metabolomics of Saccharomyces Cerevisiae Using Liquid Chromatography Coupled with Tandem Mass Spectrometry
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JoVE Journal Biyokimya
Quantitative Metabolomics of Saccharomyces Cerevisiae Using Liquid Chromatography Coupled with Tandem Mass Spectrometry

Quantitative Metabolomics of Saccharomyces Cerevisiae Using Liquid Chromatography Coupled with Tandem Mass Spectrometry

DOI:
10.3791/62061-v

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07:25 min

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January 05, 2021

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, ,
1Biyoloji Bölümü,Concordia University, 2Centre for Biological Applications of Mass Spectrometry,Concordia University

Bölümler

  • 00:04Introduction
  • 00:42Cell Transfer and Culture
  • 01:21Metabolite Extraction
  • 02:44Extracted Metabolite Separation and Analysis
  • 04:04Membrane Integrity Assessment
  • 05:24Results: Representative Metabolic Intermediate and Product Identification and Quantification
  • 06:50Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

We present a protocol for the identification and quantitation of major classes of water-soluble metabolites in the yeast Saccharomyces cerevisiae. The described method is versatile, robust, and sensitive. It allows the separation of structural isomers and stereoisomeric forms of water-soluble metabolites from each other.

Etiketler

Quantitative MetabolomicsSaccharomyces CerevisiaeLiquid ChromatographyTandem Mass SpectrometryQuenchingMetabolite ExtractionChloroformMethanolGlass BeadsVortexAqueous PhaseAcetonitrileLiquid Chromatography-tandem Mass Spectrometry

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