需要订阅 JoVE 才能查看此. 登录或开始免费试用。
Quantitative Metabolomics of Saccharomyces Cerevisiae Using Liquid Chromatography Coupled with Tandem Mass Spectrometry
Chapters
- 00:04Introduction
- 00:42Cell Transfer and Culture
- 01:21Metabolite Extraction
- 02:44Extracted Metabolite Separation and Analysis
- 04:04Membrane Integrity Assessment
- 05:24Results: Representative Metabolic Intermediate and Product Identification and Quantification
- 06:50Conclusion
We present a protocol for the identification and quantitation of major classes of water-soluble metabolites in the yeast Saccharomyces cerevisiae. The described method is versatile, robust, and sensitive. It allows the separation of structural isomers and stereoisomeric forms of water-soluble metabolites from each other.
