ジャーナル
/
生化学
/
Quantitative Metabolomics of Saccharomyces Cerevisiae Using Liquid Chromatography Coupled with Tandem Mass Spectrometry
JoVE Journal
生化学
このコンテンツを視聴するには、JoVE 購読が必要です。  サインイン又は無料トライアルを申し込む。
JoVE Journal 生化学
Quantitative Metabolomics of Saccharomyces Cerevisiae Using Liquid Chromatography Coupled with Tandem Mass Spectrometry

Quantitative Metabolomics of Saccharomyces Cerevisiae Using Liquid Chromatography Coupled with Tandem Mass Spectrometry

3,977 Views

•

07:25 min

•

January 05, 2021

DOI:

10.3791/62061-v

DOI:
10.3791/62061-v

•

07:25 min
January 05, 2021

•

8 Views
, ,
1生物学科,Concordia University, 2Centre for Biological Applications of Mass Spectrometry,Concordia University

概要

Automatically generated

We present a protocol for the identification and quantitation of major classes of water-soluble metabolites in the yeast Saccharomyces cerevisiae. The described method is versatile, robust, and sensitive. It allows the separation of structural isomers and stereoisomeric forms of water-soluble metabolites from each other.

Explore More Videos

Quantitative MetabolomicsSaccharomyces CerevisiaeLiquid ChromatographyTandem Mass SpectrometryQuenchingMetabolite ExtractionChloroformMethanolGlass BeadsVortexAqueous PhaseAcetonitrileLiquid Chromatography-tandem Mass Spectrometry

Read Article

Cite this Article

Mohammad, K., Jiang, H., Titorenko, V. I. Quantitative Metabolomics of Saccharomyces Cerevisiae Using Liquid Chromatography Coupled with Tandem Mass Spectrometry. J. Vis. Exp. (167), e62061, doi:10.3791/62061 (2021).

Download .ris file

Copy

Share Video

.

Copy