Journal
/
Biología del desarrollo
/
Defining the Program of Maternal mRNA Translation during In vitro Maturation using a Single Oocyte Reporter Assay
JoVE Revista
Biología del desarrollo
Se requiere una suscripción a JoVE para ver este contenido.  Inicie sesión o comience su prueba gratuita.
JoVE Revista Biología del desarrollo
Defining the Program of Maternal mRNA Translation during In vitro Maturation using a Single Oocyte Reporter Assay

Defining the Program of Maternal mRNA Translation during In vitro Maturation using a Single Oocyte Reporter Assay

4,170 Views

•

08:00 min

•

June 16, 2021

DOI:

10.3791/62041-v

DOI:
10.3791/62041-v

•

08:00 min
June 16, 2021

•

3 Views
, , ,
1Center for Reproductive Sciences,University of California at San Francisco, 2Department of Obstetrics Gynecology and Reproductive Sciences,University of California at San Francisco, 3Present affiliation: Laboratoire de Biologie du Développement-Institut de Biologie Paris Seine, LBD-IBPS,Sorbonne Université

Summary

Automatically generated

This protocol describes a reporter assay to study the regulation of mRNA translation in single oocytes during in vitro maturation.

Explore More Videos

Maternal MRNA TranslationIn Vitro Oocyte MaturationSingle Oocyte Reporter AssayTime-lapse MicroscopyReporter AccumulationTranslational ActivationTranslational RepressionAntral FollicleCumulus-oocyte Complex (COC)DenudingCilostamideMicroinjectionMCherryYpetTime-lapse Imaging

Read Article

Cite this Article

Costermans, N. G. J., Daldello, E. M., Marathe, R. J., Conti, M. Defining the Program of Maternal mRNA Translation during In vitro Maturation using a Single Oocyte Reporter Assay. J. Vis. Exp. (172), e62041, doi:10.3791/62041 (2021).

Download .ris file

Copy

Share Video

.

Copy