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JoVE Journal Developmental Biology
Defining the Program of Maternal mRNA Translation during In vitro Maturation using a Single Oocyte Reporter Assay
 

Defining the Program of Maternal mRNA Translation during In vitro Maturation using a Single Oocyte Reporter Assay

Article DOI: 10.3791/62041-v • 08:00 min • June 16th, 2021
June 16th, 2021 •
1,2, 1,2,3, 1,2, 1,2
1Center for Reproductive Sciences, University of California at San Francisco, 2Department of Obstetrics Gynecology and Reproductive Sciences, University of California at San Francisco, 3Present affiliation: Laboratoire de Biologie du Développement-Institut de Biologie Paris Seine, LBD-IBPS, Sorbonne Université

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Automatic Translation

This protocol describes a reporter assay to study the regulation of mRNA translation in single oocytes during in vitro maturation.

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Keywords: Maternal MRNA Translation In Vitro Oocyte Maturation Single Oocyte Reporter Assay Time-lapse Microscopy Reporter Accumulation Translational Activation Translational Repression Antral Follicle Cumulus-oocyte Complex (COC) Denuding Cilostamide Microinjection MCherry Ypet Time-lapse Imaging
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