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Defining the Program of Maternal mRNA Translation during In vitro Maturation using a Single Oocyte Reporter Assay
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Defining the Program of Maternal mRNA Translation during In vitro Maturation using a Single Oocyte Reporter Assay
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June 16, 2021
DOI:
1Center for Reproductive Sciences,University of California at San Francisco, 2Department of Obstetrics Gynecology and Reproductive Sciences,University of California at San Francisco, 3Present affiliation: Laboratoire de Biologie du Développement-Institut de Biologie Paris Seine, LBD-IBPS,Sorbonne Université
Summary
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This protocol describes a reporter assay to study the regulation of mRNA translation in single oocytes during in vitro maturation.
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Costermans, N. G. J., Daldello, E. M., Marathe, R. J., Conti, M. Defining the Program of Maternal mRNA Translation during In vitro Maturation using a Single Oocyte Reporter Assay. J. Vis. Exp. (172), e62041, doi:10.3791/62041 (2021).
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