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从鸡胚胎的直膜到研究棒到锥细胞相互作用的锥形丰富文化
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Cone-Enriched Cultures from the Retina of Chicken Embryos to Study Rod to Cone Cellular Interactions
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从鸡胚胎的直膜到研究棒到锥细胞相互作用的锥形丰富文化

DOI:
10.3791/61998-v

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08:04 min

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March 20, 2021

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, , , , ,
1Department of Genetics – Sorbonne Université, INSERM, CNRS,Institut de la Vision

Chapters

  • 00:04Introduction
  • 00:58Recovery of the Chicken Embryos
  • 02:11Dissection of the Retinas
  • 02:50Preparation of Retinal Cell Suspension
  • 03:40Retinal Cell Seeding
  • 05:16Results: Epithelium-Derived Cone Viability Factor (EdCVF), Clone 0073-09-37
  • 07:21Conclusion

Summary

Automatic Translation

Automatic Translation

我们描述了一种从鸡胚胎的网膜中获取锥体光感受器的初级培养物的方法，并将其用于高含量筛选。

Tags

Cone ReceptorsChicken EmbryoRod-cone InteractionsRetinal DegenerationCone-enriched CulturePrimary Cell CultureHamburger And Hamilton StagesCarbon Dioxide-independent MediumRinger’s MediumTrypsin

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