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In Utero Electroporation Approaches to Study the Excitability of Neuronal Subpopulations and Single-cell Connectivity
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Neuroscience
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JoVE Journal Neuroscience
In Utero Electroporation Approaches to Study the Excitability of Neuronal Subpopulations and Single-cell Connectivity

In Utero Electroporation Approaches to Study the Excitability of Neuronal Subpopulations and Single-cell Connectivity

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9,378 Views

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10:49 min

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February 15, 2017

DOI:

10.3791/55139-v

DOI:
10.3791/55139-v

•

10:49 min
February 15, 2017

•

2 Views
, , ,
1Department for Molecular and Cellular Biology,Centro Nacional de Biotecnología (CNB-CSIC), 2Molecular Neurobiology Department, Centro de Biología Molecular “Severo Ochoa”,Consejo Superior de Investigaciones Científicas (CSIC-UAM)

Summary

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This manuscript provides protocols that use in utero electroporation (IUE) to describe the structural connectivity of neurons at the single-cell level and the excitability of fluorescently labeled neurons. Histology is used to characterize dendritic and axonal projections. Whole-cell recording in acute slices is used to investigate excitability.

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Briz, C. G., Navarrete, M., Esteban, J. A., Nieto, M. In Utero Electroporation Approaches to Study the Excitability of Neuronal Subpopulations and Single-cell Connectivity. J. Vis. Exp. (120), e55139, doi:10.3791/55139 (2017).

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