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Modellazione semplice, economica e modulare delle cellule utilizzando il DNA
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February 24, 2021
DOI:
1Graduate Program in Bioengineering,University of California San Francisco and University of California Berkeley, 2Department of Pharmaceutical Chemistry,University of California San Francisco, 3Scribe Biosciences, 4Department of Bioengineering and Therapeutic Sciences,University of California San Francisco, 5California Institute for Quantitative Biosciences,University of California San Francisco, 6Chan Zuckerberg Biohub,University of California San Francisco, 7Department of Chemical & Biomolecular Engineering,University of California Berkeley, 8Helen Wills Neuroscience Institute,University of California Berkeley, 9Department of Mechanical Engineering,University of California Berkeley, 10Center for Cellular Construction,University of California San Francisco
Summary
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Qui presentiamo un protocollo alle cellule micropattern a risoluzione monocellulare utilizzando l'adesione programmata dal DNA. Questo protocollo utilizza una piattaforma di fotolitografia da banco per creare modelli di oligonucleotidi del DNA su un vetrino e quindi etichetta le membrane cellulari con oligonucleotidi complementari disponibili in commercio. L'ibridazione degli oligos si traduce in adesione cellulareprogrammata.
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Cabral, K. A., Patterson, D. M., Scheideler, O. J., Cole, R., Abate, A. R., Schaffer, D. V., Sohn, L. L., Gartner, Z. J. Simple, Affordable, and Modular Patterning of Cells using DNA. J. Vis. Exp. (168), e61937, doi:10.3791/61937 (2021).
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