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Simple, Affordable, and Modular Patterning of Cells using DNA
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Simple, Affordable, and Modular Patterning of Cells using DNA

Simple, Affordable, and Modular Patterning of Cells using DNA

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08:59 min

February 24, 2021

DOI:

08:59 min
February 24, 2021

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Summary

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Here we present a protocol to micropattern cells at single-cell resolution using DNA-programmed adhesion. This protocol uses a benchtop photolithography platform to create patterns of DNA oligonucleotides on a glass slide and then labels cell membranes with commercially available complementary oligonucleotides. Hybridization of the oligos results in programmed cell adhesion.

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