This content is Free Access.
Simple, Affordable, and Modular Patterning of Cells using DNA
3,819 Views
•
08:59 min
•
February 24, 2021
DOI:
1Graduate Program in Bioengineering,University of California San Francisco and University of California Berkeley, 2Department of Pharmaceutical Chemistry,University of California San Francisco, 3Scribe Biosciences, 4Department of Bioengineering and Therapeutic Sciences,University of California San Francisco, 5California Institute for Quantitative Biosciences,University of California San Francisco, 6Chan Zuckerberg Biohub,University of California San Francisco, 7Department of Chemical & Biomolecular Engineering,University of California Berkeley, 8Helen Wills Neuroscience Institute,University of California Berkeley, 9Makine Mühendisliği Bölümü,University of California Berkeley, 10Center for Cellular Construction,University of California San Francisco
Özet
Automatically generated
Here we present a protocol to micropattern cells at single-cell resolution using DNA-programmed adhesion. This protocol uses a benchtop photolithography platform to create patterns of DNA oligonucleotides on a glass slide and then labels cell membranes with commercially available complementary oligonucleotides. Hybridization of the oligos results in programmed cell adhesion.
Read Article
Cite this Article
Cabral, K. A., Patterson, D. M., Scheideler, O. J., Cole, R., Abate, A. R., Schaffer, D. V., Sohn, L. L., Gartner, Z. J. Simple, Affordable, and Modular Patterning of Cells using DNA. J. Vis. Exp. (168), e61937, doi:10.3791/61937 (2021).
Copy