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Simple, Affordable, and Modular Patterning of Cells using DNA
JoVE 杂志
生物工程
This content is Free Access.
JoVE 杂志 生物工程
Simple, Affordable, and Modular Patterning of Cells using DNA

Simple, Affordable, and Modular Patterning of Cells using DNA

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08:59 min

February 24, 2021

DOI:

08:59 min
February 24, 2021

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Summary

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Here we present a protocol to micropattern cells at single-cell resolution using DNA-programmed adhesion. This protocol uses a benchtop photolithography platform to create patterns of DNA oligonucleotides on a glass slide and then labels cell membranes with commercially available complementary oligonucleotides. Hybridization of the oligos results in programmed cell adhesion.

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