Here we present a protocol to micropattern cells at single-cell resolution using DNA-programmed adhesion. This protocol uses a benchtop photolithography platform to create patterns of DNA oligonucleotides on a glass slide and then labels cell membranes with commercially available complementary oligonucleotides. Hybridization of the oligos results in programmed cell adhesion.
Cabral, K. A., Patterson, D. M., Scheideler, O. J., Cole, R., Abate, A. R., Schaffer, D. V., Sohn, L. L., Gartner, Z. J. Simple, Affordable, and Modular Patterning of Cells using DNA. J. Vis. Exp. (168), e61937, doi:10.3791/61937 (2021).