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Journal
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Bioengineering
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为光遗传学实验构建简单通用的照明系统
JoVE Journal
Bioengineering
This content is Free Access.
JoVE Journal
Bioengineering
Building a Simple and Versatile Illumination System for Optogenetic Experiments
Please note that all translations are automatically generated.
Click here for the English version.
为光遗传学实验构建简单通用的照明系统
DOI:
10.3791/61914-v
•
06:41 min
•
January 12, 2021
•
Phillip Kyriakakis
,
Lourdes Fernandez de Cossio
,
Patrick Wade Howard
,
Sivleng Kouv
,
Marianne Catanho
,
Vincent J. Hu
,
Robert Kyriakakis
,
Molly E. Allen
,
Yunhan Ma
,
Marcelo Aguilar-Rivera
,
Todd P. Coleman
1
Department of Bioengineering
,
University of California, San Diego
,
2
University of California, San Diego
,
3
Department of Mathematical Computational, and Systems biology
,
University of California, Irvine
,
4
Department of Biomedical Engineering
,
Duke University
Chapters
00:05
Introduction
00:31
Electrical Circuit Construction
01:38
Light Emitting Diode (LED) Construction
05:10
Results: Representative LED-Controlled Phytochrome B (PhyB) Expression Analysis
06:06
Conclusion
Summary
Automatic Translation
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Automatic Translation
该协议描述了如何使用PhyB和PIF3进行光遗传学实验以控制红光和远红光的基因表达。其中包括构建简单灵活的照明系统的分步说明,该系统可以使用计算机控制基因表达或其他光遗传学。
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Illumination System
Optogenetic Experiments
LED
Electrical Circuit
Arduino
Soldering
Wire Connector
Resistor
Cathode
Anode
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