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Building a Simple and Versatile Illumination System for Optogenetic Experiments
JoVE 杂志
生物工程
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JoVE 杂志 生物工程
Building a Simple and Versatile Illumination System for Optogenetic Experiments

Building a Simple and Versatile Illumination System for Optogenetic Experiments

3,429 Views

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06:41 min

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January 12, 2021

DOI:

10.3791/61914-v

DOI:
10.3791/61914-v

•

06:41 min
January 12, 2021

•

13 Views
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1Department of Bioengineering,University of California, San Diego, 2University of California, San Diego, 3Department of Mathematical Computational, and Systems biology,University of California, Irvine, 4Department of Biomedical Engineering,Duke University

Summary

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This protocol describes how to perform optogenetic experiments for controlling gene expression with red and far-red light using PhyB and PIF3. Included are step-by-step instructions for building a simple and flexible illumination system, which enables the control of gene expression or other optogenetics with a computer.

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Cite this Article

Kyriakakis, P., Fernandez de Cossio, L., Howard, P. W., Kouv, S., Catanho, M., Hu, V. J., Kyriakakis, R., Allen, M. E., Ma, Y., Aguilar-Rivera, M., Coleman, T. P. Building a Simple and Versatile Illumination System for Optogenetic Experiments. J. Vis. Exp. (167), e61914, doi:10.3791/61914 (2021).

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