Rivista
/
Bioingegneria
/
Building a Simple and Versatile Illumination System for Optogenetic Experiments
JoVE Journal
Bioingegneria
This content is Free Access.
JoVE Journal Bioingegneria
Building a Simple and Versatile Illumination System for Optogenetic Experiments

Building a Simple and Versatile Illumination System for Optogenetic Experiments

3,477 Views

•

06:41 min

•

January 12, 2021

DOI:

10.3791/61914-v

DOI:
10.3791/61914-v

•

06:41 min
January 12, 2021

•

13 Views
, , , , , , , , , ,
1Department of Bioengineering,University of California, San Diego, 2University of California, San Diego, 3Department of Mathematical Computational, and Systems biology,University of California, Irvine, 4Department of Biomedical Engineering,Duke University

Summary

Automatically generated

This protocol describes how to perform optogenetic experiments for controlling gene expression with red and far-red light using PhyB and PIF3. Included are step-by-step instructions for building a simple and flexible illumination system, which enables the control of gene expression or other optogenetics with a computer.

Explore More Videos

Illumination SystemOptogenetic ExperimentsLEDElectrical CircuitArduinoSolderingWire ConnectorResistorCathodeAnodeSolder PasteShrink TubeFlux

Read Article

Cite this Article

Kyriakakis, P., Fernandez de Cossio, L., Howard, P. W., Kouv, S., Catanho, M., Hu, V. J., Kyriakakis, R., Allen, M. E., Ma, Y., Aguilar-Rivera, M., Coleman, T. P. Building a Simple and Versatile Illumination System for Optogenetic Experiments. J. Vis. Exp. (167), e61914, doi:10.3791/61914 (2021).

Download .ris file

Copy

Share Video

.

Copy