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De afleiding van eiwitkristallen met I3C met behulp van Random Microseed Matrix Screening
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
Derivatization of Protein Crystals with I3C using Random Microseed Matrix Screening
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De afleiding van eiwitkristallen met I3C met behulp van Random Microseed Matrix Screening

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4,436 Views

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14:04 min

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January 16, 2021

DOI:

10.3791/61894-v

DOI:
10.3791/61894-v

•

14:04 min
January 16, 2021

•

6 Views
, , ,
1School of Biological Sciences,The University of Adelaide, 2Institute of Photonics and Advanced Sensing (IPAS), School of Biological Sciences,The University of Adelaide

Summary

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Dit artikel presenteert een methode om eiwitkristallen te genereren die zijn afgeleid met I3C (5-amino-2,4,6-triiodoisoftaalzuur) met behulp van microseeding om nieuwe kristallisatieomstandigheden in schaarse matrixschermen te genereren. De trays kunnen worden opgezet met behulp van vloeibare doseerrobots of met de hand.

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Cite this Article

Truong, J. Q., Nguyen, S., Bruning, J. B., Shearwin, K. E. Derivatization of Protein Crystals with I3C using Random Microseed Matrix Screening. J. Vis. Exp. (167), e61894, doi:10.3791/61894 (2021).

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