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Derivatization of Protein Crystals with I3C using Random Microseed Matrix Screening
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
Derivatization of Protein Crystals with I3C using Random Microseed Matrix Screening

Derivatization of Protein Crystals with I3C using Random Microseed Matrix Screening

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4,435 Views

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14:04 min

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January 16, 2021

DOI:

10.3791/61894-v

DOI:
10.3791/61894-v

•

14:04 min
January 16, 2021

•

6 Views
, , ,
1School of Biological Sciences,The University of Adelaide, 2Institute of Photonics and Advanced Sensing (IPAS), School of Biological Sciences,The University of Adelaide

Summary

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This article presents a method to generate protein crystals derivatized with I3C (5-amino-2,4,6-triiodoisophthalic acid) using microseeding to generate new crystallization conditions in sparse matrix screens. The trays can be set up using liquid dispensing robots or by hand.

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Cite this Article

Truong, J. Q., Nguyen, S., Bruning, J. B., Shearwin, K. E. Derivatization of Protein Crystals with I3C using Random Microseed Matrix Screening. J. Vis. Exp. (167), e61894, doi:10.3791/61894 (2021).

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