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Derivatization of Protein Crystals with I3C using Random Microseed Matrix Screening
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Derivatization of Protein Crystals with I3C using Random Microseed Matrix Screening

Derivatization of Protein Crystals with I3C using Random Microseed Matrix Screening

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14:04 min

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January 16, 2021

DOI:

10.3791/61894-v

DOI:
10.3791/61894-v

•

14:04 min
January 16, 2021

•

6 Views
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1School of Biological Sciences,The University of Adelaide, 2Institute of Photonics and Advanced Sensing (IPAS), School of Biological Sciences,The University of Adelaide

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This article presents a method to generate protein crystals derivatized with I3C (5-amino-2,4,6-triiodoisophthalic acid) using microseeding to generate new crystallization conditions in sparse matrix screens. The trays can be set up using liquid dispensing robots or by hand.

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Truong, J. Q., Nguyen, S., Bruning, J. B., Shearwin, K. E. Derivatization of Protein Crystals with I3C using Random Microseed Matrix Screening. J. Vis. Exp. (167), e61894, doi:10.3791/61894 (2021).

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