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Derivatization of Protein Crystals with I3C using Random Microseed Matrix Screening
JoVE Journal
Bioquímica
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JoVE Journal Bioquímica
Derivatization of Protein Crystals with I3C using Random Microseed Matrix Screening

Derivatization of Protein Crystals with I3C using Random Microseed Matrix Screening

DOI:
10.3791/61894-v

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14:04 min

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January 16, 2021

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, , ,
1School of Biological Sciences,The University of Adelaide, 2Institute of Photonics and Advanced Sensing (IPAS), School of Biological Sciences,The University of Adelaide

Capítulos

  • 00:00Introduction
  • 01:51Preparation of 13C Stock
  • 02:24Addition of I3C to Protein Stock
  • 03:18Making a Rounded Probe
  • 04:04Making a Seed Stock
  • 06:33Setting Up an rMMS Screen
  • 08:08Data Processing
  • 11:21Representative Results
  • 13:22Conclusion

Summary

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This article presents a method to generate protein crystals derivatized with I3C (5-amino-2,4,6-triiodoisophthalic acid) using microseeding to generate new crystallization conditions in sparse matrix screens. The trays can be set up using liquid dispensing robots or by hand.

Tags

Keywords: Protein CrystalDerivatizationI3CRandom Microseed Matrix ScreeningX-ray CrystallographyPhasing MoleculeAnomalous SignalSingle Wavelength Anomalous Discussion PhasingLithium I3CProtein CrystallizationStructure Solution

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