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Knoglemarvstransplantationsprocedurer hos mus til undersøgelse af klonisk hæmatopoiesis
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Immunology and Infection
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JoVE Journal Immunology and Infection
Bone Marrow Transplantation Procedures in Mice to Study Clonal Hematopoiesis

Knoglemarvstransplantationsprocedurer hos mus til undersøgelse af klonisk hæmatopoiesis

DOI:
10.3791/61875-v

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08:00 min

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May 26, 2021

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, , , , , , , ,
1Department of Biochemistry and Molecular Genetics,University of Virginia School of Medicine, 2Department of Cardiology, Xinqiao Hospital,Army Medical University, 3Department of Cardiology,Osaka City University Graduate School of Medicine, 4Hematovascular Biology Center, Robert M. Berne Cardiovascular Research Center,University of Virginia School of Medicine

Chapters

  • 00:05Introduction
  • 00:49Partially Shielded Irradiation
  • 02:17Bone Isolation
  • 03:31Bone Marrow Isolation
  • 04:40Bone Marrow Cell Transplantation
  • 05:22Results: Representative Donor and Recipient Cell Expansion After BMT
  • 06:57Conclusion

Summary

Automatic Translation

Automatic Translation

Vi beskriver tre metoder til knoglemarvstransplantation (BMT): BMT med total kropsbestråling, BMT med afskærmet bestråling og BMT-metode uden prækonditionering (adoptiv BMT) til undersøgelse af klonisk hæmatopoiesis i musemodeller.

Tags

Keywords: Bone Marrow TransplantationClonal HematopoiesisTotal Body RadiationThorax And Abdomen ShieldingHead ShieldingBone IsolationMouse Model

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