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Immunology and Infection
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Live Imaging of Chemokine Receptors in Zebrafish Neutrophils During Wound Responses
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Live Imaging of Chemokine Receptors in Zebrafish Neutrophils During Wound Responses

Live Imaging of Chemokine Receptors in Zebrafish Neutrophils During Wound Responses

DOI:
10.3791/61679-v

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06:48 min

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December 04, 2020

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, , , ,
1Department of Physiology, Development and Neuroscience,University of Cambridge

Chapters

  • 00:04Introduction
  • 01:04Larval Ventral Fin Wounding and Live Imaging Preparation
  • 02:22Live Confocal Imaging
  • 03:03Zebrafish Neutrophil Receptor Internalization Quantification
  • 04:36Results: Representative Differential CXCR1 and CXCR2 Wound Response Dynamics
  • 06:12Conclusion

Summary

Automatic Translation

Automatic Translation

Here we describe protocols to perform live imaging and quantitative analysis of chemoattractant receptor dynamics in zebrafish neutrophils

Tags

Keywords: ZebrafishNeutrophilsWound ResponseChemokine ReceptorsLive ImagingIn VivoInflammationImmune ResponseTransgenicFin WoundingConfocal MicroscopyTime-lapse ImagingReceptor Internalization

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