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Yüksek Çözünürlüklü Erime Analizi Kullanarak CALR Geninde Genetik Varyant Tespiti
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Genetics
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JoVE Journal Genetics
Genetic Variant Detection in the CALR gene using High Resolution Melting Analysis
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Yüksek Çözünürlüklü Erime Analizi Kullanarak CALR Geninde Genetik Varyant Tespiti

Türkçe

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4,430 Views

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08:46 min

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August 26, 2020

DOI:

10.3791/61642-v

DOI:
10.3791/61642-v

•

08:46 min
August 26, 2020

•

12 Views
, , , , , ,
1Department of Hematology,University Medical Centre Ljubljana, 2Clinical Institute for Genomic Medicine,University Medical Centre Ljubljana, 3Faculty of Pharmacy,University of Ljubljana

Summary

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Yüksek çözünürlüklü erime analizi (HRM), genetik varyant tespiti için hassas ve hızlı bir çözümdür. Erime eğrisinin şeklini değiştiren heteroduplexes ile sonuçlanan sıra farklılıklarına bağlıdır. HRM ve agarose jel elektroforezi taranarak, indels gibi farklı genetik varyantlar tanımlanabilir.

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Pajič, T., Belčič Mikič, T., Podgornik, H., Klun, J., Šućurović, S., Zver, S., Sever, M. Genetic Variant Detection in the CALR gene using High Resolution Melting Analysis. J. Vis. Exp. (162), e61642, doi:10.3791/61642 (2020).

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