このコンテンツを視聴するには、JoVE 購読が必要です。 サインイン又は無料トライアルを申し込む。
Genetic Variant Detection in the CALR gene using High Resolution Melting Analysis
4,430 Views
•
08:46 min
•
August 26, 2020
DOI:
概要
Automatically generated
High resolution melting analysis (HRM) is a sensitive and rapid solution for genetic variant detection. It depends on sequence differences that result in heteroduplexes changing the shape of the melting curve. By combing HRM and agarose gel electrophoresis, different types of genetic variants such as indels can be identified.
Explore More Videos
Genetic Variant DetectionCALR GeneHigh Resolution Melting AnalysisMyeloproliferative DisordersEssential ThrombocythemiaPrimary MyelofibrosisQPCR HRM Master MixNegative ControlsPositive ControlsNo Template Control (NTC)Optical Adhesive FilmDissociation AnalysisAmplification PlotFluorescence ReadingsPassive Reference
Read Article
Cite this Article
Pajič, T., Belčič Mikič, T., Podgornik, H., Klun, J., Šućurović, S., Zver, S., Sever, M. Genetic Variant Detection in the CALR gene using High Resolution Melting Analysis. J. Vis. Exp. (162), e61642, doi:10.3791/61642 (2020).
Copy