Waiting
Elaborazione accesso...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Genetics / Genetic Variant Detection in the CALR gene using…
JoVE Journal
Genetics

È necessario avere un abbonamento a JoVE per visualizzare questo Contenuto. Accedi o inizia la tua prova gratuita.

JoVE Journal Genetics
Genetic Variant Detection in the CALR gene using High Resolution Melting Analysis
 

Genetic Variant Detection in the CALR gene using High Resolution Melting Analysis

Article DOI: 10.3791/61642-v • 08:46 min • August 26th, 2020
August 26th, 2020 •
1,2, 1, 1,3, 1, 1, 1, 1
1Department of Hematology, University Medical Centre Ljubljana, 2Clinical Institute for Genomic Medicine, University Medical Centre Ljubljana, 3Faculty of Pharmacy, University of Ljubljana

Capitoli

Riepilogo
Automatic Translation

High resolution melting analysis (HRM) is a sensitive and rapid solution for genetic variant detection. It depends on sequence differences that result in heteroduplexes changing the shape of the melting curve. By combing HRM and agarose gel electrophoresis, different types of genetic variants such as indels can be identified.

Tags

Genetic Variant Detection CALR Gene High Resolution Melting Analysis Calreticulin Mutations Hematologic Condition Non-hematologic Conditions Somatic Genetic Variants Hotspot Region Myeloproliferative Disorders Essential Thrombocythemia Primary Myelofibrosis QPCR HRM Master Mix Replicates DNA Sample Control Negative Controls Positive Controls Samples
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter