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牛早期蚁卵泡卵母细胞的体外培养策略
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
In Vitro Culture Strategy for Oocytes from Early Antral Follicle in Cattle
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牛早期蚁卵泡卵母细胞的体外培养策略

DOI:
10.3791/61625-v

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09:30 min

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July 08, 2020

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, , ,
1Reproductive and Developmental Biology Laboratory, Department of Health, Animal Science and Food Safety,University of Milan

Chapters

  • 00:04Introduction
  • 00:50Media Preparation
  • 01:44Ovary Collection and Processing
  • 03:03Selection and Isolation of the Follicles and Retrieval of the COCs
  • 04:31Long in Vitro Culture of the Oocytes (long IVCO
  • 07:15Results: Meiotic Progression of Oocytes after long IVCO
  • 08:52Conclusion

Summary

Automatic Translation

Automatic Translation

我们描述了在发育的早期阶段从卵巢卵泡分离生长的卵母细胞的过程，以及建立体外培养系统，该系统可以支持生长和分化，一次，一次，一次，一个发育阶段。

Tags

In Vitro CultureOocytesEarly Antral FolliclesCattleGenetic SalvageThreatened SpeciesLocal BreedsOvary SlicingFollicle SelectionCulture MediumCilostamideLong IVCO Medium96-well PlateIncubation Conditions

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