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In Vitro Culture Strategy for Oocytes from Early Antral Follicle in Cattle
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Developmental Biology
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JoVE Journal Developmental Biology
In Vitro Culture Strategy for Oocytes from Early Antral Follicle in Cattle

In Vitro Culture Strategy for Oocytes from Early Antral Follicle in Cattle

English

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8,178 Views

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09:30 min

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July 08, 2020

DOI:

10.3791/61625-v

DOI:
10.3791/61625-v

•

09:30 min
July 08, 2020

•

10 Views
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1Reproductive and Developmental Biology Laboratory, Department of Health, Animal Science and Food Safety,University of Milan

Summary

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We describe the procedures for isolation of growing oocytes from ovarian follicles at early stages of development, as well as the setup of an in vitro culture system which can support the growth and differentiation up to the fully-grown stage.  

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Cite this Article

Barros, R. G., Lodde, V., Franciosi, F., Luciano, A. M. In Vitro Culture Strategy for Oocytes from Early Antral Follicle in Cattle. J. Vis. Exp. (161), e61625, doi:10.3791/61625 (2020).

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