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In Vitro Culture Strategy for Oocytes from Early Antral Follicle in Cattle
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发育生物学
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JoVE 杂志 发育生物学
In Vitro Culture Strategy for Oocytes from Early Antral Follicle in Cattle

In Vitro Culture Strategy for Oocytes from Early Antral Follicle in Cattle

8,179 Views

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09:30 min

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July 08, 2020

DOI:

10.3791/61625-v

DOI:
10.3791/61625-v

•

09:30 min
July 08, 2020

•

10 Views
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1Reproductive and Developmental Biology Laboratory, Department of Health, Animal Science and Food Safety,University of Milan

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We describe the procedures for isolation of growing oocytes from ovarian follicles at early stages of development, as well as the setup of an in vitro culture system which can support the growth and differentiation up to the fully-grown stage.  

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Barros, R. G., Lodde, V., Franciosi, F., Luciano, A. M. In Vitro Culture Strategy for Oocytes from Early Antral Follicle in Cattle. J. Vis. Exp. (161), e61625, doi:10.3791/61625 (2020).

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