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マウス腹腔内脂肪貯蔵所からの脂肪形成性および線維炎症性間質細胞亜集団の単離
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Immunology and Infection
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JoVE Journal Immunology and Infection
Isolation of Adipogenic and Fibro-Inflammatory Stromal Cell Subpopulations from Murine Intra-Abdominal Adipose Depots
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マウス腹腔内脂肪貯蔵所からの脂肪形成性および線維炎症性間質細胞亜集団の単離

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2,307 Views

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06:50 min

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August 16, 2020

DOI:

10.3791/61610-v

DOI:
10.3791/61610-v

•

06:50 min
August 16, 2020

•

3 Views
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1Diabetes Pharmacology,Novo Nordisk A/S, 2Touchstone Diabetes Center, Department of Internal Medicine,University of Texas Southwestern Medical Center

Summary

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このプロトコルは、蛍光活性化細胞選別または免疫磁気ビーズ分離により、マウス腹腔内白色脂肪組織(WAT)デポから脂肪形成および線維炎症性間質細胞亜集団を単離するための技術的アプローチを説明しています。

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Cite this Article

Peics, J., Vishvanath, L., Zhang, Q., Shan, B., Pedersen, T. Å., Gupta, R. K. Isolation of Adipogenic and Fibro-Inflammatory Stromal Cell Subpopulations from Murine Intra-Abdominal Adipose Depots. J. Vis. Exp. (162), e61610, doi:10.3791/61610 (2020).

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