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Immunologie und Infektion
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Isolation of Adipogenic and Fibro-Inflammatory Stromal Cell Subpopulations from Murine Intra-Abdominal Adipose Depots
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Immunologie und Infektion
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JoVE Journal Immunologie und Infektion
Isolation of Adipogenic and Fibro-Inflammatory Stromal Cell Subpopulations from Murine Intra-Abdominal Adipose Depots

Isolation of Adipogenic and Fibro-Inflammatory Stromal Cell Subpopulations from Murine Intra-Abdominal Adipose Depots

DOI:
10.3791/61610-v

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06:50 min

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August 16, 2020

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, , , , ,
1Diabetes Pharmacology,Novo Nordisk A/S, 2Touchstone Diabetes Center, Department of Internal Medicine,University of Texas Southwestern Medical Center

Kapitel

  • 00:00Introduction
  • 00:35Adipocyte Precursor Cell (APC) and Fibro‐Inflammatory Progenitor (FIP) FACS Isolation
  • 02:22Adipogenic and Non‐Adipogenic Cell Fraction Immunomagnetic Bead Separation
  • 04:34Cell Culture and Differentiation
  • 05:07Results: Isolation and Differentiation of Adipogenic Stromal Cell Subset
  • 06:11Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

This protocol describes the technical approach to isolate adipogenic and fibro-inflammatory stromal cell subpopulations from murine intra-abdominal white adipose tissue (WAT) depots by fluorescence-activated cell sorting or immunomagnetic bead separation.

Tags

Immunologie und InfektionStromal CellsSVFPDGFRFIPsAPCsFACSImmunomagnetic Beads

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