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Isolation of Adipogenic and Fibro-Inflammatory Stromal Cell Subpopulations from Murine Intra-Abdominal Adipose Depots
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Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Isolation of Adipogenic and Fibro-Inflammatory Stromal Cell Subpopulations from Murine Intra-Abdominal Adipose Depots

Isolation of Adipogenic and Fibro-Inflammatory Stromal Cell Subpopulations from Murine Intra-Abdominal Adipose Depots

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06:50 min

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August 16, 2020

DOI:

10.3791/61610-v

DOI:
10.3791/61610-v

•

06:50 min
August 16, 2020

•

3 Views
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1Diabetes Pharmacology,Novo Nordisk A/S, 2Touchstone Diabetes Center, Department of Internal Medicine,University of Texas Southwestern Medical Center

Summary

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This protocol describes the technical approach to isolate adipogenic and fibro-inflammatory stromal cell subpopulations from murine intra-abdominal white adipose tissue (WAT) depots by fluorescence-activated cell sorting or immunomagnetic bead separation.

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Immunologia e infezioniStromal CellsSVFPDGFRFIPsAPCsFACSImmunomagnetic Beads

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Cite this Article

Peics, J., Vishvanath, L., Zhang, Q., Shan, B., Pedersen, T. Å., Gupta, R. K. Isolation of Adipogenic and Fibro-Inflammatory Stromal Cell Subpopulations from Murine Intra-Abdominal Adipose Depots. J. Vis. Exp. (162), e61610, doi:10.3791/61610 (2020).

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