Journal
/
免疫与感染
/
Isolation of Adipogenic and Fibro-Inflammatory Stromal Cell Subpopulations from Murine Intra-Abdominal Adipose Depots
JoVE 杂志
免疫与感染
需要订阅 JoVE 才能查看此.  登录或开始免费试用。
JoVE 杂志 免疫与感染
Isolation of Adipogenic and Fibro-Inflammatory Stromal Cell Subpopulations from Murine Intra-Abdominal Adipose Depots

Isolation of Adipogenic and Fibro-Inflammatory Stromal Cell Subpopulations from Murine Intra-Abdominal Adipose Depots

2,307 Views

•

06:50 min

•

August 16, 2020

DOI:

10.3791/61610-v

DOI:
10.3791/61610-v

•

06:50 min
August 16, 2020

•

3 Views
, , , , ,
1Diabetes Pharmacology,Novo Nordisk A/S, 2Touchstone Diabetes Center, Department of Internal Medicine,University of Texas Southwestern Medical Center

Summary

Automatically generated

This protocol describes the technical approach to isolate adipogenic and fibro-inflammatory stromal cell subpopulations from murine intra-abdominal white adipose tissue (WAT) depots by fluorescence-activated cell sorting or immunomagnetic bead separation.

Explore More Videos

免疫与感染Stromal CellsSVFPDGFRFIPsAPCsFACSImmunomagnetic Beads

Read Article

Cite this Article

Peics, J., Vishvanath, L., Zhang, Q., Shan, B., Pedersen, T. Å., Gupta, R. K. Isolation of Adipogenic and Fibro-Inflammatory Stromal Cell Subpopulations from Murine Intra-Abdominal Adipose Depots. J. Vis. Exp. (162), e61610, doi:10.3791/61610 (2020).

Download .ris file

Copy

Share Video

.

Copy