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Constructing Mutanten in Serotype 1 Streptococcus pneumoniae stam 519/43
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Immunology and Infection
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JoVE Journal Immunology and Infection
Constructing Mutants in Serotype 1 Streptococcus pneumoniae strain 519/43
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Constructing Mutanten in Serotype 1 Streptococcus pneumoniae stam 519/43

Nederlands

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1,507 Views

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06:06 min

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September 11, 2020

DOI:

10.3791/61594-v

DOI:
10.3791/61594-v

•

06:06 min
September 11, 2020

•

2 Views
, , , ,
1Faculty of Infections and Tropical Diseases, Department of Infection Biology,London School of Hygiene and Tropical Medicine, 2Division of Medicine,University College London, 3Division of Infection and Immunity,University College London UCL

Summary

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Hier beschrijven we een S. pneumoniae serotype 1 stam 519/43 die genetisch kan worden gemodificeerd door gebruik te maken van zijn vermogen om op natuurlijke wijze DNA en een zelfmoordplasmide te verkrijgen. Als proof of principle werd een isogene mutant in het pneumolysine (ply) gen gemaakt.

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Cite this Article

Terra, V. S., Plumptre, C. D., Wall, E. C., Brown, J. S., Wren, B. W. Constructing Mutants in Serotype 1 Streptococcus pneumoniae strain 519/43. J. Vis. Exp. (163), e61594, doi:10.3791/61594 (2020).

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