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Konstruktion von Mutanten im Serotyp 1 Streptococcus pneumoniae Stamm 519/43
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Immunology and Infection
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JoVE Journal Immunology and Infection
Constructing Mutants in Serotype 1 Streptococcus pneumoniae strain 519/43
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Konstruktion von Mutanten im Serotyp 1 Streptococcus pneumoniae Stamm 519/43

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1,507 Views

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06:06 min

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September 11, 2020

DOI:

10.3791/61594-v

DOI:
10.3791/61594-v

•

06:06 min
September 11, 2020

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2 Views
, , , ,
1Faculty of Infections and Tropical Diseases, Department of Infection Biology,London School of Hygiene and Tropical Medicine, 2Division of Medicine,University College London, 3Division of Infection and Immunity,University College London UCL

Summary

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Hier beschreiben wir einen S. pneumoniae Serotyp 1 Stamm 519/43, der genetisch verändert werden kann, indem er seine Fähigkeit nutzt, DNA und ein Suizid-Plasmid auf natürliche Weise zu erwerben. Als Prinzipienbeweis wurde eine isogene Mutante im Pneumolysin-Gen (Ply) hergestellt.

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Cite this Article

Terra, V. S., Plumptre, C. D., Wall, E. C., Brown, J. S., Wren, B. W. Constructing Mutants in Serotype 1 Streptococcus pneumoniae strain 519/43. J. Vis. Exp. (163), e61594, doi:10.3791/61594 (2020).

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