/
/
Constructing Mutants in Serotype 1 Streptococcus pneumoniae strain 519/43
Se requiere una suscripción a JoVE para ver este contenido. Inicie sesión o comience su prueba gratuita.
Constructing Mutants in Serotype 1 Streptococcus pneumoniae strain 519/43
1,507 Views
•
06:06 min
•
September 11, 2020
DOI:
Summary
Automatically generated
Here, we describe a S. pneumoniae serotype 1 strain 519/43 that can be genetically modified by using its ability to naturally acquire DNA and a suicide-plasmid. As proof of principle, an isogenic mutant in the pneumolysin (ply) gene was made.
Read Article
Cite this Article
Terra, V. S., Plumptre, C. D., Wall, E. C., Brown, J. S., Wren, B. W. Constructing Mutants in Serotype 1 Streptococcus pneumoniae strain 519/43. J. Vis. Exp. (163), e61594, doi:10.3791/61594 (2020).
Copy