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Propagation of the Microsporidian Parasite Edhazardia aedis in Aedes aegypti Mosquitoes
JoVE 杂志
免疫与感染
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JoVE 杂志 免疫与感染
Propagation of the Microsporidian Parasite Edhazardia aedis in Aedes aegypti Mosquitoes

Propagation of the Microsporidian Parasite Edhazardia aedis in Aedes aegypti Mosquitoes

DOI:
10.3791/61574-v

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05:29 min

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August 13, 2020

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, , , ,
1Department of Entomology,The Ohio State University, 2USDA/ARS Center for Medical, Agricultural, and Veterinary Entomology

Chapters

  • 00:04Conclusion
  • 00:39Days 0 1: Hatch Ae. aegypti Eggs Infected with E. aedis
  • 01:23Days 4-5: Hatch Uninfected Ae. aegypti
  • 01:49Days 7 8: Horizontal Transmission
  • 03:45Results: Effective E. aedis Infection in Filial Generation
  • 05:00Conclusion

Summary

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自动翻译

A protocol to culture the microsporidian parasite Edhazardia aedis. The parasite is passaged from one generation of Aedes aegypti mosquitoes to the next via horizontal transfer at the larval stage followed by vertical transmission at the adult stage. Live sporoplasms survive long-term in infected eggs.

Tags

Keywords: MicrosporidianEdhazardia AedisAedes AegyptiParasite PropagationMosquito RearingSpore QuantificationLarval InfectionSpore InoculationLarval Food Slurry

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