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Propagation of the Microsporidian Parasite Edhazardia aedis で Aedes aegypti Mosquitoes
JoVE Journal
免疫学と感染
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JoVE Journal 免疫学と感染
Propagation of the Microsporidian Parasite Edhazardia aedis で Aedes aegypti Mosquitoes

Propagation of the Microsporidian Parasite Edhazardia aedis で Aedes aegypti Mosquitoes

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05:29 min

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August 13, 2020

DOI:

10.3791/61574-v

DOI:
10.3791/61574-v

•

05:29 min
August 13, 2020

•

7 Views
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1昆虫学科,The Ohio State University, 2USDA/ARS Center for Medical, Agricultural, and Veterinary Entomology

概要

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A protocol to culture the microsporidian parasite Edhazardia aedis. The parasite is passaged from one generation of Aedes aegypti mosquitoes to the next via horizontal transfer at the larval stage followed by vertical transmission at the adult stage. Live sporoplasms survive long-term in infected eggs.

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Cite this Article

Grigsby, A., Kelly, B. J., Sanscrainte, N. D., Becnel, J. J., Short, S. M. Propagation of the Microsporidian Parasite Edhazardia aedis で Aedes aegypti Mosquitoes. J. Vis. Exp. (162), e61574, doi:10.3791/61574 (2020).

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