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Inmunología y contagio
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Propagation of the Microsporidian Parasite Edhazardia aedis de Aedes aegypti Mosquitoes
JoVE Revista
Inmunología y contagio
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JoVE Revista Inmunología y contagio
Propagation of the Microsporidian Parasite Edhazardia aedis de Aedes aegypti Mosquitoes

Propagation of the Microsporidian Parasite Edhazardia aedis de Aedes aegypti Mosquitoes

DOI:
10.3791/61574-v

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05:29 min

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August 13, 2020

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, , , ,
1Department of Entomology,The Ohio State University, 2USDA/ARS Center for Medical, Agricultural, and Veterinary Entomology

Capítulos

  • 00:04Conclusion
  • 00:39Days 0 1: Hatch Ae. aegypti Eggs Infected with E. aedis
  • 01:23Days 4-5: Hatch Uninfected Ae. aegypti
  • 01:49Days 7 8: Horizontal Transmission
  • 03:45Results: Effective E. aedis Infection in Filial Generation
  • 05:00Conclusion

Summary

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A protocol to culture the microsporidian parasite Edhazardia aedis. The parasite is passaged from one generation of Aedes aegypti mosquitoes to the next via horizontal transfer at the larval stage followed by vertical transmission at the adult stage. Live sporoplasms survive long-term in infected eggs.

Tags

MicrosporidianEdhazardia AedisAedes AegyptiParasite PropagationMosquito RearingSpore QuantificationLarval InfectionSpore InoculationLarval Food Slurry

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