Journal
/
Chemistry
/
Eletroretinograma acoplado direto (DC-ERG) para gravar as respostas elétricas evocadas pela luz do epitélio pigmento de retina do rato
JoVE Journal
Chemistry
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Chemistry
Direct-Coupled Electroretinogram (DC-ERG) for Recording the Light-Evoked Electrical Responses of the Mouse Retinal Pigment Epithelium

Eletroretinograma acoplado direto (DC-ERG) para gravar as respostas elétricas evocadas pela luz do epitélio pigmento de retina do rato

português

Automatically Generated

4,953 Views

•

09:10 min

•

July 14, 2020

DOI:

10.3791/61491-v

DOI:
10.3791/61491-v

•

09:10 min
July 14, 2020

•

19 Views
, , , ,
1Retinal Neurophysiology Section, National Eye Institute,National Institutes of Health, 2Ocular and Stem Cell Translational Research Section, National Eye Institute,National Institutes of Health, 3Human Visual Function Core, National Eye Institute,National Institutes of Health

Summary

Automatically generated

Aqui, apresentamos um método para registrar respostas elétricas evocadas pela luz do epitélio pigmento da retina (RPE) em camundongos usando uma técnica conhecida como DC-ERGs descrita pela primeira vez por Marmorstein, Peachey, e colegas no início dos anos 2000.

Explore More Videos

Direct-coupled ElectroretinogramDC-ERGRetinal Pigment EpitheliumNon-invasive EvaluationAge-related ChangesDisease ProgressionPharmacological InterventionReproducibilityCapillary ElectrodeHBSSVacuum ChamberCustom StandMicroelectrode HolderT-clipMagnetic Ball JointElectrode TestGround ElectrodeReference Electrode

Read Article

Cite this Article

Miyagishima, K. J., Zhang, C., Malechka, V. V., Bharti, K., Li, W. Direct-Coupled Electroretinogram (DC-ERG) for Recording the Light-Evoked Electrical Responses of the Mouse Retinal Pigment Epithelium. J. Vis. Exp. (161), e61491, doi:10.3791/61491 (2020).

Download .ris file

Copy

Share Video

.

Copy