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Direct-Coupled Electroretinogram (DC-ERG) for Recording the Light-Evoked Electrical Responses of the Mouse Retinal Pigment Epithelium
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Chemistry
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JoVE Journal Chemistry
Direct-Coupled Electroretinogram (DC-ERG) for Recording the Light-Evoked Electrical Responses of the Mouse Retinal Pigment Epithelium

Direct-Coupled Electroretinogram (DC-ERG) for Recording the Light-Evoked Electrical Responses of the Mouse Retinal Pigment Epithelium

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4,953 Views

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09:10 min

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July 14, 2020

DOI:

10.3791/61491-v

DOI:
10.3791/61491-v

•

09:10 min
July 14, 2020

•

19 Views
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1Retinal Neurophysiology Section, National Eye Institute,National Institutes of Health, 2Ocular and Stem Cell Translational Research Section, National Eye Institute,National Institutes of Health, 3Human Visual Function Core, National Eye Institute,National Institutes of Health

Summary

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Here, we present a method for recording light-evoked electrical responses of the retinal pigment epithelium (RPE) in mice using a technique known as DC-ERGs first described by Marmorstein, Peachey, and colleagues in the early 2000s.

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Miyagishima, K. J., Zhang, C., Malechka, V. V., Bharti, K., Li, W. Direct-Coupled Electroretinogram (DC-ERG) for Recording the Light-Evoked Electrical Responses of the Mouse Retinal Pigment Epithelium. J. Vis. Exp. (161), e61491, doi:10.3791/61491 (2020).

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