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Direct-Coupled Electroretinogram (DC-ERG) for Recording the Light-Evoked Electrical Responses of the Mouse Retinal Pigment Epithelium
 

Direct-Coupled Electroretinogram (DC-ERG) for Recording the Light-Evoked Electrical Responses of the Mouse Retinal Pigment Epithelium

Article DOI: 10.3791/61491-v • 09:10 min • July 14th, 2020
July 14th, 2020 •
1, *2, *3, 2, 1
1Retinal Neurophysiology Section, National Eye Institute, National Institutes of Health, 2Ocular and Stem Cell Translational Research Section, National Eye Institute, National Institutes of Health, 3Human Visual Function Core, National Eye Institute, National Institutes of Health
* These authors contributed equally

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Here, we present a method for recording light-evoked electrical responses of the retinal pigment epithelium (RPE) in mice using a technique known as DC-ERGs first described by Marmorstein, Peachey, and colleagues in the early 2000s.

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Direct-Coupled Electroretinogram DC-ERG Mouse Retinal Pigment Epithelium Retinal Pigment Epithelium Function Age-related Changes Disease Progression Pharmacological Intervention Effects Capillary Electrode Preparation Ohm Software Application Syringe Needle Silicone Rubber Gasket Degassed HBSS Threaded Cap Electrode Holder Capillary Electrodes Vacuum Chamber Microelectro
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