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Live-Cell Forward Genetic Approach to Identify and Isolate Developmental Mutants in Chlamydia trachomatis
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Immunology and Infection
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JoVE Journal Immunology and Infection
Live-Cell Forward Genetic Approach to Identify and Isolate Developmental Mutants in Chlamydia trachomatis

Live-Cell Forward Genetic Approach to Identify and Isolate Developmental Mutants in Chlamydia trachomatis

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4,115 Views

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10:32 min

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June 10, 2020

DOI:

10.3791/61365-v

DOI:
10.3791/61365-v

•

10:32 min
June 10, 2020

•

2 Views
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1Department of Biological Sciences,University of Idaho

Summary

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This protocol utilizes fluorescent promoter-reporters, live-cell microscopy, and individual inclusion extraction in a directed forward genetic approach to identify and isolate developmental mutants of Chlamydia trachomatis.

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Chlamydia TrachomatisLive-cellForward Genetic ApproachDevelopmental MutantsReporterMutagenesisEMSHost Cell CultureInfectionIsolation

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Chiarelli, T. J., Grieshaber, N. A., Grieshaber, S. S. Live-Cell Forward Genetic Approach to Identify and Isolate Developmental Mutants in Chlamydia trachomatis. J. Vis. Exp. (160), e61365, doi:10.3791/61365 (2020).

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