/
/
Live-Cell Forward Genetic Approach to Identify and Isolate Developmental Mutants in Chlamydia trachomatis
È necessario avere un abbonamento a JoVE per visualizzare questo. Accedi o inizia la tua prova gratuita.
Live-Cell Forward Genetic Approach to Identify and Isolate Developmental Mutants in Chlamydia trachomatis
4,115 Views
•
10:32 min
•
June 10, 2020
DOI:
Summary
Automatically generated
This protocol utilizes fluorescent promoter-reporters, live-cell microscopy, and individual inclusion extraction in a directed forward genetic approach to identify and isolate developmental mutants of Chlamydia trachomatis.
Read Article
Cite this Article
Chiarelli, T. J., Grieshaber, N. A., Grieshaber, S. S. Live-Cell Forward Genetic Approach to Identify and Isolate Developmental Mutants in Chlamydia trachomatis. J. Vis. Exp. (160), e61365, doi:10.3791/61365 (2020).
Copy