This protocol utilizes fluorescent promoter-reporters, live-cell microscopy, and individual inclusion extraction in a directed forward genetic approach to identify and isolate developmental mutants of Chlamydia trachomatis.
Chiarelli, T. J., Grieshaber, N. A., Grieshaber, S. S. Live-Cell Forward Genetic Approach to Identify and Isolate Developmental Mutants in Chlamydia trachomatis. J. Vis. Exp. (160), e61365, doi:10.3791/61365 (2020).