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Live-Cell Forward Genetic Approach to Identify and Isolate Developmental Mutants in Chlamydia trachomatis
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Live-Cell Forward Genetic Approach to Identify and Isolate Developmental Mutants in Chlamydia trachomatis

Live-Cell Forward Genetic Approach to Identify and Isolate Developmental Mutants in Chlamydia trachomatis

DOI:
10.3791/61365-v

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10:32 min

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June 10, 2020

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, ,
1Department of Biological Sciences,University of Idaho

Capítulos

  • 00:04Introduction
  • 00:30Chlamydia trachomatis (Ctr) Reporter Mutagenesis
  • 01:34Mutant Ctr Imaging
  • 03:09Microscope Setup and Imaging
  • 04:25Altered Developmental Mutagenized Chlamydia Phenotype Identification and Isolation
  • 07:07Mutant Isolate Phenotype Verification
  • 08:06Results: Representative Ctr Developmental Mutant Identification
  • 09:52Conclusion

Summary

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This protocol utilizes fluorescent promoter-reporters, live-cell microscopy, and individual inclusion extraction in a directed forward genetic approach to identify and isolate developmental mutants of Chlamydia trachomatis.

Tags

Chlamydia TrachomatisLive-cellForward Genetic ApproachDevelopmental MutantsReporterMutagenesisEMSHost Cell CultureInfectionIsolation

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